Journal: bioRxiv
Article Title: Intraventricular infusion to circumvent the blood-brain barrier to gemcitabine
doi: 10.64898/2026.05.01.722145
Figure Lengend Snippet: GB cells were exposed to GEM for 1h or 24 h at the indicated concentrations. After GEM exposure, cells were maintained in drug-free fresh culture medium for 72 h before a viability assay with Crystal Violet. IC50 of GEM on the A172, U87-MG and U118-MG human GB cell lines were indicated in the legend of . Phase contrast microscopy images of GB cells treated for 24 h at IC 90. Cells were maintained in culture in drug free medium and observed 24 h or 5 days after GEM treatment. Note the presence of enlarged / flattened cells.
Article Snippet: Human GB cell lines A172, U87-MG and U118-MG were purchased from American Type Culture Collection (ATCC) (LGC Standards, Strasbourg, France) and cultured in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (Jacques Boy, Reims, France), 2 mM glutamine and penicillin / streptomycin.
Techniques: Viability Assay, Microscopy